ck7  (Servicebio Inc)

Journal: BMC Cancer

Article Title: Subtyping atypical eosinophilic renal cell tumours through integrated morphological, immunohistochemical, and mutational characters

doi: 10.1186/s12885-026-15870-1

Figure Lengend Snippet: Histopathological and immunohistochemical features of atypical eosinophilic renal cell tumours. A1–A6: Renal oncocytoma. A1: The tumour is well-circumscribed from the surrounding tissue and unencapsulated. A2: The tumour exhibits solid and nested/sheet-like growth with intervening edematous stroma. A3: CK7 demonstrates focal strong cytoplasmic positive. A4: CD117 shows diffuse weak cytoplasmic positive. A5: CK20 is negative. A6: Colloidal iron staining is negative. B1–B6: Eosinophilic chromophobe renal cell carcinoma. B1: The tumour is relatively well-circumscribed, without involvement of the renal sinus fat. B2: Solidly packed tumour cells include distinct populations with botryoid morphology and eosinophilic cytoplasm. B3: CK7 shows diffuse strong cytoplasmic positive. B4: CD117 shows diffuse strong cytoplasmic positive. B5: CK20 is negative. B6: Colloidal iron staining is positive. C1–C6: Low-grade oncocytic tumour. C1: The tumour is well-circumscribed and unencapsulated. C2: Solid and acinar growth patterns are observed. C3: CK7 shows diffuse strong cytoplasmic positive. C4: CD117 is negative. C5: CK20 is negative. C6: Colloidal iron staining is negative. D1–D6: Eosinophilic vacuolated tumour. D1: The tumour is relatively well-circumscribed from the adjacent normal tissue, with scattered proliferative blood vessels within the tumour. D2: The tumour is arranged in solid nests and cords. D3: CK7 shows focal weak cytoplasmic positive. D4: CD117 shows diffuse moderate cytoplasmic positive. D5: CK20 is negative. D6: Colloidal iron staining is negative. E1–E6: Eosinophilic solid and cystic renal cell carcinoma. E1: The tumour is well-circumscribed, unencapsulated, and exhibits a cystic and solid structure. E2: The tumour shows cystic and solid growth patterns. E3: CK7 is negative. E4: CD117 is negative. E5: CK20 shows focal strong cytoplasmic positive. E6: Colloidal iron staining is negative. F1–F6: Normal renal tissue (Control). F1: Normal renal tissue, showing renal tubules, glomeruli, and thick-walled vessels. F2: Normal renal parenchyma. F3: CK7 shows diffuse strong cytoplasmic positive. F4: CD117 shows diffuse strong cytoplasmic positive. D5: CK20 is negative. F6: Colloidal iron staining is negative. Hematoxylin and eosin (H&E) staining x20 (A1-F1) and x40 (A2-F2). EnVision for CK7, CD117 and CK20 × 200. Colloidal iron staining x200

Article Snippet: The antibodies used in the study are CK7 (EP16, Origene, Wuxi, China), CK20 (EP23, Origene, Wuxi, China), CD117 (EP10, Origene, Wuxi, China), mTOR (ab109268, Abcam, Shanghai, China), Vimentin (UMAB159, Origene, Wuxi, China), CAIX(H-11, Origene, Wuxi, China), SDHB (OTI1H6, Origene, Wuxi, China), FH (OTI1F10, Origene, Wuxi, China), Cathepsin K (ab37259, Abcam, Shanghai, China) and Pax8 (OTI6H8, Origene, Wuxi, China).

Techniques: Immunohistochemical staining, Staining, Control

Journal: PLOS One

Article Title: Patient-derived organoids predict chemotherapy response of locally advanced gastric cancer

doi: 10.1371/journal.pone.0339416

Figure Lengend Snippet: (A) Schematic overview of GC PDOs experimental workflow: tissue isolation, organoid culture, histopathological evaluation, genomic analysis, in vitro drug sensitivity testing, and prediction of clinical response. (B) Representative bright-field images of GC-010 PDOs from day 0 to day 9 post-seeding in Matrigel. Scale bars, 100 µm. Similar growth patterns were observed across all 17 established PDO lines. (C) Bright-field images highlighting the morphological diversity among PDOs derived from SRCC (GC-014, GC-027) and non-SRCC (GC-010, GC-011). Scale bars, 100 µm. (D) Representative H&E staining images of GC PDOs and their corresponding parental tumors. GC-007 is SRCC and GC-025 is non-SRCC. Black arrow indicates signet ring cells; blue arrow indicates solid or cystic structures. Scale bars, 50 µm. (E) IHC staining of Ki67, CK7, CK20 and CDX2 in GC PDOs and their parental tumors from GC-007 and GC-025. GC PDOs faithfully recapitulated histologic features of their parental tumors. Scale bars, 50 µm. P, passage. D, day. GC, gastric cancer. PDOs, patient-derived organoids. SRCC, signet-ring cell carcinoma. Non-SRCC, non-signet-ring cell carcinoma.

Article Snippet: The primary antibodies used for IHC included Ki67 (clone SP6, 1:600 dilution, Servicebio, Wuhan, China), CK7 (clone 7D8G8, 1:600 dilution, Servicebio, Wuhan, China), CDX2 (clone 4H2A8, 1:1500 dilution, Servicebio, Wuhan, China), and CK20 (clone SP33, 1:1500 dilution, Servicebio, Wuhan, China).

Techniques: Isolation, In Vitro, Derivative Assay, Staining, Immunohistochemistry